Sharp PC-9300 Technical Information PDF herunterladen (Seite 14)

1 Instrument

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maximum volume per well is 250 µl. Overfilling a well will result in spills and possible

damage to the instrument.

Delay: The number of seconds between the injection of the reagent and taking a

measurement. Setting a delay after the injection will allow flash-type luminescence to fully

actualize before a reading. The minimum delay value is 0.5 seconds in 0.1-second

increments.

Wavelength: Selects one or two Absorbance filters from a list.

Optical Kit: Selects a Fluorescence Optical Kit from a list.

Reading: The number of times a sample plate is read. The number of reads can be set

between 1 and 99.

Period: The time interval between readings of the same well ranging from 1 to 120

minutes in 1-minute increments.

Prime: The injector system is wetted and filled with the reagent.

Flush: The protocol used to clean the injector system after a run. The recommended

flush protocol consists of steps for washing with deionized water, 70% ethanol, deionized

water, and then air drying.

Reverse Purge: The protocol retrieves any unused reagent from the injector system.

After a run, this protocol can be used to push the reagent back into the reagent bottle to

cut down on waste.

Preset Protocols: A set of popular assay protocols preloaded into the instrument for

user convenience. These protocols cannot be renamed or deleted. A user-modified

version can be saved by touching the USER button on the PROTOCOL screen.

User Protocols: Protocols created by the user which can be retrieved later. All

parameters including the plate well mapping are saved. These protocols can be modified,

renamed, or deleted.

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Sharp PC-9300 Technical Information Seite 14