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APPENDIX

Appendix A - Specifications

General Specifications

• Detection Modes: Luminescence, Fluorescence, and Absorbance

• Sample Format: 96-well microplate

• Read Format: 1-96 samples in 96-well microplate

• Integrated PC Operating System: Windows CE

• User Interface: 6.6 in VGA color TFT LCD display with touch screen

• Data Output: PC & Mac compatible .csv file format

• PC requirements (optional): Windows XP operating system or higher

• Dimensions: 53 cm D x 44 cm W x 31 cm H ( 21 in D x 17.3 in W x 12.2 in H)

• Weight: ~16 kg (~ 35 lbs)

• Power Source: 100-240 VAC, 50/60 Hz

• Operating Temperature: 15-30°C (60 - 85°F)

• Operating Humidity: 5-75% non-condensing

• Warranty: 1 year, parts and labor

• Regulatory: CE, for research use only

Luminescence Module Specifications

• Detector: head-on photon counting photomultiplier tube (PMT)

• Wavelength Range: 350 to 650 nm

• Detection Limit: 3x10

-21

moles of luciferase

• Linear Dynamic Range: >8 logs

• Cross-Talk: 1 million RLUs using 96-well plates

- White plates: 10

-6

(for the Corning



Costar



product #3789)

-4

(for the Greiner Bio-One Lumitrac 200)

- Black plates: < 10

-6

Absorbance Module Specifications

• Light Source: LED

• Detector: large-area photodiode

• Spectral Range: 360 - 800 nm

• Filter Wheel Capacity: holds up to six filters. Includes four installed filters and two empty filter holders for

user configuration.

• Wavelengths Available: 450, 560, 600, 750 nm

• Photometric Measuring Range: 0 - 4.0 OD

• Linear Dynamic Range: 0 - 4.0 OD

• OD Accuracy: <2.0% at 4.0 OD typical

• OD Precision: <0.2% from 0 - 4.0 OD

Fluorescence Module Specifications

• Light Source: wavelength matched LED

• Detector: PiN-photodiode

• Read Position: top reading

• Wavelength Selection: snap-in optical kits

• Wavelengths Available:

- UV (Ex: 365 nm, Em: 410 - 460 nm)

- Blue (Ex: 490 nm, Em: 510 - 570 nm)

- Green (Ex: 525 nm, Em: 580 - 640 nm)

- Red (Ex: 625 nm, Em: 660 - 720 nm)

- Custom kits upon request

• Detection Limit: 0.5 fmol/200 µl, or 1 ppt of fluorescein in 96-well microplate

• Linear Dynamic Range: 6 decades (assay dependent)

1 2 ... 54 55 56 57 58 59 60 61 62 63 64 ... 67 68

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